Barcelona defender Samuel Umtiti sustained a calf injury on his return to individual training, the Spanish La Liga club said in a statement on Saturday.
The 26-year-old French centre back has struggled with knee and foot issues in the last few seasons and Barcelona said there was no time frame for his return.
Football in Spain has been suspended due to the Covid-19 pandemic since March. La Liga is hoping to start matches again in June, although it has yet to confirm dates for fixtures.
Players have started to train in small groups and will progress to full sessions before resuming games.
Barcelona lead the table with 11 matches remaining and hold a two-point lead over Real Madrid, who are due to begin training on Monday.
